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eclipse ti e c2 confocal laser scanning microscope  (Nikon)


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    Structured Review

    Nikon eclipse ti e c2 confocal laser scanning microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Confocal Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti+microscope/C2%2B/pmc12811640-78-16-23
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 confocal laser scanning microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.
    Figure Legend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Techniques Used: Staining, Mutagenesis, Fluorescence, Microscopy

    Related Articles

    Microscopy:

    Article Title: c-di-GMP-Dependent Regulation of Motility by comFB and comFC
    Article Snippet: .. Images were collected using a Nikon Eclipse Ti microscope equipped with an Orca Flash 4.0 Digital camera (Hamamatsu) with a Nikon TIRF 1.45 NA Plan Neofluor 100 oil immersion objective. .. NIS-Elements AR (v 4.40, Nikon) software was used for image acquisition.

    Article Title: Non-canonical role of natural quinones in mitochondrial nucleoid organization for maintaining respiration and protecting cardiac function
    Article Snippet: For fixed and immunostained cells, super-resolution imaging was performed using the Nikon Super-Resolution Microscope System (N-SIM). .. The samples were observed under an Eclipse Ti microscope (Nikon) equipped with a CFI Apochromat TIRF 100XC immersion objective (numerical aperture 1.49) using 561 and 488 nm emission lasers. .. Obtained SIM images were reconstructed in NIS-Elements Advanced Research software (Nikon).

    Article Title: Aurora B maintains spherical shape of mitotic cells via simultaneously stabilizing myosin II and vimentin.
    Article Snippet: .. Time-lapse images were captured every 5 min using a 20× objective lens on an Eclipse Ti microscope (Nikon). ..

    Article Title: [2508.11518] Measurement of tissue viscosity to relate force and motion in collective cell migration
    Article Snippet: .. An Eclipse Ti microscope (Nikon Instruments) was used with a 4× or 10× objective and an Orca Flash 4.0 digital camera (Hamamatsu) running Elements Ar software (Nikon). ..

    Article Title: Tumor-associated macrophages promote chemoresistance to Paclitaxel via activating NOTCH2-JAG1 juxtacrine signaling.
    Article Snippet: The medium was then replaced with phenol red-free L-15 medium (Invitrogen/Thermo Fisher Scientific, MA, USA) containing 10% FBS and 1% P/S. .. Time-lapse imaging was performed at 5-min intervals using a 20× objective lens on an Eclipse Ti microscope (Nikon, Tokyo, Japan). ..

    Article Title: Traction and Stress Control Formation and Motion of +1/2 Topological Defects in Epithelial Cell Monolayers
    Article Snippet: .. Time lapse imaging of cells and fluorescent particles was performed using an Eclipse Ti microscope (Nikon Instruments, Melville, NY) with a 10× numerical aperture 0.3 objective or a 20× numerical aperture 0.5 objective (Nikon) and an Orca Flash 4.0 camera (Hamamatsu, Bridgewater, NJ) controlled by Elements Ar software (Nikon). ..

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist.
    Article Snippet: Results were normalized to total cell 2 protein contents, which were collected using the DC Protein Assay Kit from Bio-Rad. .. 3 4 Sub-plasma membrane Ca2+ imaging and analysis of total cell cAMP levels 5 Measurements of the cytoplasmic Ca2+ concentration beneath the plasma membrane 6 were made with Fluo-5F in a total internal reflection fluorescence (TIRF) setup 7 consisting of an Eclipse Ti microscope with a 60× 1.45 oil-immersion NA-objective 8 (Nikon), using 491 nm laser excitation (Cobolt AB, Solna, Sweden) and fluorescence 9 detection at 530 nm (35 nm half band-width) with an Orca-ER CCD camera 10 (Hamamatsu, Japan). .. Total cell cAMP contents were analyzed using a cAMP Enzyme 11 Immunoassay Kit according to the instructions of the manufacturer (Sigma Aldrich).

    Article Title: Measurement of tissue viscosity to relate force and motion in collective cell migration
    Article Snippet: .. An Eclipse Ti microscope (Nikon Instruments) was used with a 4× or 10× objective and an Orca Flash 4.0 digital camera (Hamamatsu) running Elements Ar software (Nikon). ..

    Software:

    Article Title: [2508.11518] Measurement of tissue viscosity to relate force and motion in collective cell migration
    Article Snippet: .. An Eclipse Ti microscope (Nikon Instruments) was used with a 4× or 10× objective and an Orca Flash 4.0 digital camera (Hamamatsu) running Elements Ar software (Nikon). ..

    Article Title: Traction and Stress Control Formation and Motion of +1/2 Topological Defects in Epithelial Cell Monolayers
    Article Snippet: .. Time lapse imaging of cells and fluorescent particles was performed using an Eclipse Ti microscope (Nikon Instruments, Melville, NY) with a 10× numerical aperture 0.3 objective or a 20× numerical aperture 0.5 objective (Nikon) and an Orca Flash 4.0 camera (Hamamatsu, Bridgewater, NJ) controlled by Elements Ar software (Nikon). ..

    Article Title: Measurement of tissue viscosity to relate force and motion in collective cell migration
    Article Snippet: .. An Eclipse Ti microscope (Nikon Instruments) was used with a 4× or 10× objective and an Orca Flash 4.0 digital camera (Hamamatsu) running Elements Ar software (Nikon). ..

    Imaging:

    Article Title: Tumor-associated macrophages promote chemoresistance to Paclitaxel via activating NOTCH2-JAG1 juxtacrine signaling.
    Article Snippet: The medium was then replaced with phenol red-free L-15 medium (Invitrogen/Thermo Fisher Scientific, MA, USA) containing 10% FBS and 1% P/S. .. Time-lapse imaging was performed at 5-min intervals using a 20× objective lens on an Eclipse Ti microscope (Nikon, Tokyo, Japan). ..

    Article Title: Traction and Stress Control Formation and Motion of +1/2 Topological Defects in Epithelial Cell Monolayers
    Article Snippet: .. Time lapse imaging of cells and fluorescent particles was performed using an Eclipse Ti microscope (Nikon Instruments, Melville, NY) with a 10× numerical aperture 0.3 objective or a 20× numerical aperture 0.5 objective (Nikon) and an Orca Flash 4.0 camera (Hamamatsu, Bridgewater, NJ) controlled by Elements Ar software (Nikon). ..

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist.
    Article Snippet: Results were normalized to total cell 2 protein contents, which were collected using the DC Protein Assay Kit from Bio-Rad. .. 3 4 Sub-plasma membrane Ca2+ imaging and analysis of total cell cAMP levels 5 Measurements of the cytoplasmic Ca2+ concentration beneath the plasma membrane 6 were made with Fluo-5F in a total internal reflection fluorescence (TIRF) setup 7 consisting of an Eclipse Ti microscope with a 60× 1.45 oil-immersion NA-objective 8 (Nikon), using 491 nm laser excitation (Cobolt AB, Solna, Sweden) and fluorescence 9 detection at 530 nm (35 nm half band-width) with an Orca-ER CCD camera 10 (Hamamatsu, Japan). .. Total cell cAMP contents were analyzed using a cAMP Enzyme 11 Immunoassay Kit according to the instructions of the manufacturer (Sigma Aldrich).

    Membrane:

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist.
    Article Snippet: Results were normalized to total cell 2 protein contents, which were collected using the DC Protein Assay Kit from Bio-Rad. .. 3 4 Sub-plasma membrane Ca2+ imaging and analysis of total cell cAMP levels 5 Measurements of the cytoplasmic Ca2+ concentration beneath the plasma membrane 6 were made with Fluo-5F in a total internal reflection fluorescence (TIRF) setup 7 consisting of an Eclipse Ti microscope with a 60× 1.45 oil-immersion NA-objective 8 (Nikon), using 491 nm laser excitation (Cobolt AB, Solna, Sweden) and fluorescence 9 detection at 530 nm (35 nm half band-width) with an Orca-ER CCD camera 10 (Hamamatsu, Japan). .. Total cell cAMP contents were analyzed using a cAMP Enzyme 11 Immunoassay Kit according to the instructions of the manufacturer (Sigma Aldrich).

    Concentration Assay:

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist.
    Article Snippet: Results were normalized to total cell 2 protein contents, which were collected using the DC Protein Assay Kit from Bio-Rad. .. 3 4 Sub-plasma membrane Ca2+ imaging and analysis of total cell cAMP levels 5 Measurements of the cytoplasmic Ca2+ concentration beneath the plasma membrane 6 were made with Fluo-5F in a total internal reflection fluorescence (TIRF) setup 7 consisting of an Eclipse Ti microscope with a 60× 1.45 oil-immersion NA-objective 8 (Nikon), using 491 nm laser excitation (Cobolt AB, Solna, Sweden) and fluorescence 9 detection at 530 nm (35 nm half band-width) with an Orca-ER CCD camera 10 (Hamamatsu, Japan). .. Total cell cAMP contents were analyzed using a cAMP Enzyme 11 Immunoassay Kit according to the instructions of the manufacturer (Sigma Aldrich).

    Clinical Proteomics:

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist.
    Article Snippet: Results were normalized to total cell 2 protein contents, which were collected using the DC Protein Assay Kit from Bio-Rad. .. 3 4 Sub-plasma membrane Ca2+ imaging and analysis of total cell cAMP levels 5 Measurements of the cytoplasmic Ca2+ concentration beneath the plasma membrane 6 were made with Fluo-5F in a total internal reflection fluorescence (TIRF) setup 7 consisting of an Eclipse Ti microscope with a 60× 1.45 oil-immersion NA-objective 8 (Nikon), using 491 nm laser excitation (Cobolt AB, Solna, Sweden) and fluorescence 9 detection at 530 nm (35 nm half band-width) with an Orca-ER CCD camera 10 (Hamamatsu, Japan). .. Total cell cAMP contents were analyzed using a cAMP Enzyme 11 Immunoassay Kit according to the instructions of the manufacturer (Sigma Aldrich).

    Fluorescence:

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist.
    Article Snippet: Results were normalized to total cell 2 protein contents, which were collected using the DC Protein Assay Kit from Bio-Rad. .. 3 4 Sub-plasma membrane Ca2+ imaging and analysis of total cell cAMP levels 5 Measurements of the cytoplasmic Ca2+ concentration beneath the plasma membrane 6 were made with Fluo-5F in a total internal reflection fluorescence (TIRF) setup 7 consisting of an Eclipse Ti microscope with a 60× 1.45 oil-immersion NA-objective 8 (Nikon), using 491 nm laser excitation (Cobolt AB, Solna, Sweden) and fluorescence 9 detection at 530 nm (35 nm half band-width) with an Orca-ER CCD camera 10 (Hamamatsu, Japan). .. Total cell cAMP contents were analyzed using a cAMP Enzyme 11 Immunoassay Kit according to the instructions of the manufacturer (Sigma Aldrich).



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    Image Search Results


    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy